Solutions for Life Science & Diagnostics
Anti-β-Catenin mAb
Additional Information
Anti-β-Catenin mAb Specifications
| Background: | ï¢-Catenin, a vertebrate homologue of Drosophila armadillo is encoded by the CTNNB1 gene, and has two functions in the cell. First, it acts as a mediator of cell-cell adhesion by association with E-cadherin with the cytoskeleton. Second, it acts as a downstream transcriptional activator of Wnt signaling which plays important role in embryonic development, in the induction of cell polarity, and in the determination of cell fate. In the absence of Wnt signaling, any free ï¢-catenin is phosphorylated by glycogen synthase kinase-3ï¢ï€ GSK-3ï¢ï€© in the amino terminus of ï¢-catenin. This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated ï¢-catenin degradation via the ubiquitin-proteosome pathway. Wnt signaling stabilizes ï¢-catenin by inhibiting the activity of GSK-3ï¢ï€¬ï€ and preventing ï¢-catenin degradation. ï¢-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef) which promote gene expression of a variety of target genes including c-myc and cyclin D1. |
| Clonality: | Monoclonal |
| Clone Number: | 12F7 |
| Formulation: | 1.0 mg/mL in PBS/50% glycerol, pH 7.2 |
| Gene ID Human: | 1499 |
| Gene ID Mouse: | 12387 |
| Host Species: | Mouse |
| Immunogen: | purified maltose binding protein/N terminal half of &_eta;-catenin fusion protein |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG1 |
| Product Type: | Primary Antibody |
| Shipping: | 4 |
| Size: | 100 μg |
| Species Reactivities: | Human, Mouse, Rat |
| Status: | RUO |
| Storage: | -20℃ |
| Target: | β-catenin |
Applications: IH, IP, WB
| IHC: | 1-5 ug/mL (Heat treatment is necessary for paraffi |
| IPP: | 2 ug/200-300 uL of cell extract |
| WB: | 1 ug/mL (for chemiluminescence detection system) |
There are no references for Anti-β-Catenin mAb at this time.
Product Images
