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Product Catalog |
Cdh-1/Hct1 Monoclonal Antibody : Trial SizeCode No: K0085-3S
Target:
Cdh1 Background:
Two highly conserved WD40-repeat proteins, Cdc20 and Cdh1/Hct1, have been identified as limiting, substrate-specific regulators for anaphase-promoting complex (APC)-dependent proteolysis. Cdc20 and Cdh1 bind directly to APC and activate its cyclin ubiquitination activity. Cdc20 activates APC at the onset of anaphase, while Cdh1 activates APC from late anaphase through G1. Phosphorylation of Cdh1 by cyclin dependent kinases (Cdks), such as Cdc28, blocked Cdh1-APC interaction and APC activation, while dephosphorylation by the phosphatase Cdc14 restored this activity. Activation of the APC and cyclin degradation is required for exit from mitosis; hence, the phosphorylation of Cdc20 and Cdh1 by Cdks regulate APC activity and may control the precise progression of mitosis.
Size:
10 µg
Application:
WB
Application:
IPP
Clone Number:
DCS-266
Concentration:
0.2 mg/mL
Product Type:
Primary Antibody
Host Species:
Mouse
Species Reactivities:
Human
Species Reactivities:
Mouse
Species Reactivities:
Rat
Isotype:
IgG
Storage Temp. (°C):
4
Shipping Temp. (°C):
4 Pictures:
 References:
1) Sorensen, C.S., et al. Mol. Cell. Biol. 21, 3692-3703 (2001) Formulation:
10 g IgG in 50 L volume of PBS containing 1% BSA, 10% glycerol and 0.1% sodium azide as preservative. Sodium azide has been reported to form potentially explosive lead or copper azide in plumbing. To prevent forming of these compounds, flush drains enough after disposing of solutions containing sodium azide.
Reactivity:
This antibody reacts with human, mouse and rat Cdh1.
Intended Use:
For Research use only. Not for use in diagnostic procedure. |
