| Background: | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent Caspase activity. |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Product Type: | Kit |
| Shipping: | 4 |
| Size: | 200 assays |
| Status: | RUO |
| Storage: | -20 |
| Target: | Caspase-4 |
Applications:
There are no references for Caspase-4 Colorimetric Assay Kit at this time.