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caspase-4 Fluorometric Assay Kit

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JM-K126-100 caspase-4 Fluorometric Assay Kit 100 assays. $474.08

Additional Information

caspase-4 Fluorometric Assay Kit Specifications

Background:Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (_¬max = 400 nm); upon cleavage of the substrate by caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (_¬max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.
Clone Number:N/A
Host Species:N/A
Regulatory Statement:For Research Use Only. Not for use in diagnostic procedures.
Product Type:Kit
Size:100 assays.


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