- Diagnostic Products
- Research Products
|Background:||Stimulation of responsive cells with IFN a/b induces the formation of a transcription complex known as ISGF3. This complex binds to the interferon-stimulated response element (ISRE), thereby activating the transcription of responsive genes. The ISGF3 complex consists of tyrosine phosphorylated -STAT1a, -STAT1b (p84) and -STAT2 proteins, as well as a 48 kDa (p48) DNA-binding protein that specifically binds to the IFN-stimulated response element. Formation of this complex and its migration into the nucleus is dependent upon tyrosine phosphorylation of STAT1a/b ï€ and STAT2. Stimulation of cells with IFN-Î³ results in tyrosine phosphorylation of STAT1a (p91) but not of STAT2. Phosphorylation of STAT1a results in homodimerization and migration in to the nucleus, where it binds to the IFN-Î³ activated site (GAS). The STAT1a and STAT1b isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1b lacks 38 C-terminal amino acids. Contained within these 38 terminal amino acids of STAT1a is a critical serine residue (Ser727) whose phosphorylation is required for maximal transcription induced by IFN- Î³.|
|Regulatory Statement:||For Research Use Only. Not for use in diagnostic procedures.|
|Product Type:||Primary Antibody|
|Species Reactivities:||H, M, R|
Applications: WB, ELISA, IH
There are no references for STAT1alpha Monoclonal Antibody at this time.