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Anti-Np95 (Uhrf1) (Mouse) mAb

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D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb 100 µg/100 µL $295.00

Additional Information

Anti-Np95 (Uhrf1) (Mouse) mAb Specifications

Alternative Names:ICBP90, RNF106, AL022808
Background:DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs that appear after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, a tandem Tudor domain, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
Clonality:Monoclonal
Clone Number:Th-10a
Conjugate:Unlabeled
Formulation:1 mg/mL in PBS/50% glycerol, pH 7.2
Gene ID Mouse:18140
Host Species:Rat
Immunogen:Mouse thymic lymphoma
Regulatory Statement:For Research Use Only. Not for use in diagnostic procedures.
Isotype:IgG2aκ
Product Type:Primary Antibody
Shipping:4
Size:100 µg/100 µL
Species Reactivities:Mouse
Status:RUO
Storage:-20
Target:NP95

Applications: WB,IPP,FCM,ICC,IHC

FCM:reported.
ICC:2 µg/mL
IHC:reported.
IPP:reported.
WB:1 µg/mL
  1. Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995) (PMID:8634372)Application: IP/FCM/IHC
  2. Fujimori A et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mamm Genome. 9, 1032-5 (1998) (PMID:9880673)Application: WB
  3. Uemura T et al. Temporal and spatial localization of novel nuclear protein NP95 in mitotic and meiotic cells. Cell Struct Funct. 25, 149-59 (2000) (PMID:10984098)Application: ICC
  4. Bonapace IM et al. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. J Cell Biol. 157, 909-14 (2002) (PMID:12058012)Application: WB/ICC
  5. Muto M et al. Targeted disruption of Np95 gene renders murine embryonic stem cells hypersensitive to DNA damaging agents and DNA replication blocks. J Biol Chem. 277, 34549-55 (2002) (PMID:12084726)Application: WB
  6. Sharif J et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 450, 908-12 (2007) (PMID:17994007)Application: ICC
  7. Ohno R et al. A replication-dependent passive mechanism modulates DNA demethylation in mouse primordial germ cells. Development 140, 2892-903 (2013) (PMID:23760957)Application: ICC

 

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Anti-Np95 (Uhrf1) (Mouse) mAb