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Anti-MFG-E8 (Mouse) mAb

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D199-3 Anti-MFG-E8 (Mouse) mAb 100 µL: 1 mg/mL $255.00

Additional Information

Anti-MFG-E8 (Mouse) mAb Specifications

Alternative Names:P47, MP47, Mfgm, SED1, lactadherin
Background:Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and binds to phosphatidylserine exposed on the cell surface of apoptotic cells, then binds strongly to cells expressing avb3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.
Clonality:Monoclonal
Clone Number:18A2-G10
Conjugate:Unlabeled
Formulation:1.0 mg/mL in PBS/50% glycerol, pH 7.2
Gene ID Mouse:17304
Host Species:Hamster
Immunogen:Recombinant Mouse MFG-E8
Regulatory Statement:For Research Use Only. Not for use in diagnostic procedures.
Isotype:IgG
Product Type:Primary Antibody
Shipping:4
Size:100 µL: 1 mg/mL
Species Reactivities:Mouse
Status:RUO
Storage:-20
Target:MFG-E8

Applications: WB,IHC

IHC:1 µg/mL (Can be used in cryosections.)
WB:1 µg/mL (for chemiluminescence detection sys
Western Blotting
  1. Bobrie A et al. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles. 1, 18397 (2012) (PMID:24009879)
  2. Aziz M et al. Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2. J Immunol. 189, 393-402 (2012) (PMID:22634615)
  3. Hoffhines AJ et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem. 284, 3096-105 (2009) (PMID:19047058)
  4. Aziz MM et al. Prolactin induces MFG-E8 production in macrophages via transcription factor C/EBPbeta-dependent pathway. Apoptosis. 13, 609-20 (2008) (PMID:18392683)

Immunohistochemistry
  1. Abe T et al. Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol. 193, 1383-91 (2014) (PMID:24958900)
  2. Middendorp S et al. Mice deficient for CD137 ligand are predisposed to develop germinal center-derived B-cell lymphoma. Blood. 114, 2280-9 (2009) (PMID:19608748)
  3. Kranich J et al. Follicular dendritic cells control engulfment of apoptotic bodies by secreting Mfge8. J Exp Med. 205, 1293-302 (2008) (PMID:18490487)
  4. Yoshino M et al. Analysis of capturing skin antigens in the steady state using milk fat globule EGF factor 8-deficient skin-hyperpigmented mice. Immunol Lett. 115, 131-37 (2008) (PMID:18083239)
  5. Jinushi M et al. MFG-E8-mediated uptake of apoptotic cells by APCs links the pro- and antiinflammatory activities of GM-CSF. J Clin Invest. 117, 1902-13 (2007) (PMID:17557120)

 

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Anti-MFG-E8 (Mouse) mAb