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Product Catalog |
Topoisomerase II α monoclonal, pre-diluted : Trial SizeCode No: D081-1S
Target:
DNA Topoisomerase Iialpha Background:
Topoisomerase II (Topo II) is a nuclear enzyme that regulates the topological states of DNA by transient breakage and rejoining double-stranded DNA, catalyzing the decatenation and unknotting of topologically linked DNA circles and the relaxation of supercoiled DNA. In mammalian cells, Topo II consists of two isozymes, Topo II (170 kDa) and Topo II (180 kDa). Expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo II is expressed constantly throughout cell cycle, whereas the expression of Topo II is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. It is considered that Topo II plays an essential role in cell proliferation, especially during late S to M phase.
Size:
50 µl
Application:
IPP
Application:
IHC
Application:
WB
Clone Number:
8D2
Product Type:
Primary Antibody
Host Species:
Mouse
Species Reactivities:
Human
Isotype:
IgG2b
Storage Temp. (°C):
4
Shipping Temp. (°C):
4 References:
1) Sakaguchi, A., and Kikuchi A., J. Cell Sci. 117, 1047-1054 (2004) 2) Okuwaki, M., et al., Mol. Biol. Cell 13, 2016-2030 (2002) 3) Ogawara, Y., et al., J. Biol. Chem. 277, 21843-21850 (2002) 4) Sugimoto, K., et al., Blood 91, 1407-1417 (1998) 5) Kimura, K., et al., J. Biol. Chem. 271, 21439-21445 (1996) 6) Nakajima, T., et al., J. Biol. Chem. 271, 24842-24849 (1996) 7) Nakajima, T., et al., Oncogene 10, 651-662 (1995) 8) Kimura, K., et al., J. Biol. Chem. 269, 24523-24526 (1994) Formulation:
Culture supernatant containing 0.09% NaN3 as preservative.*Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain.
Reactivity:
This antibody reacts with human Topo II (170 kDa) on Western blotting, ImmunoprecIPPitation, and Immunohistochemistry. The epitope of 8D2 exists between amino acids 1260 and 1460 of human Topo II.
Intended Use:
For Research use only. Not for use in diagnostic procedure. |
