Solutions for Life Science & Diagnostics
Anti-DNA Topoisomerase IIα (Human) mAb
Additional Information
Anti-DNA Topoisomerase IIα (Human) mAb Specifications
| Background: | Topoisomerase II (Topo II) is a nuclear enzyme that regulates the topological states of DNA by transient breakage and rejoining double-stranded DNA, catalyzing the decatenation and unknotting of topologically linked DNA circles and the relaxation of supercoiled DNA. In mammalian cells, Topo II consists of two isozymes, Topo IIï¡ (170 kDa) and Topo IIï¢ (180 kDa). Expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo IIï¢ is expressed constantly throughout cell cycle, whereas the expression of Topo IIï¡ is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. It is considered that Topo IIï¡ plays an essential role in cell proliferation, especially during late S to M phase. |
| Clonality: | Monoclonal |
| Clone Number: | 8D2 |
| Formulation: | Prepared from culture supernatant with preservative (0.09% sodium azide) |
| Gene ID Human: | 7153 |
| Gene ID Mouse: | 21973 |
| Host Species: | Mouse |
| Immunogen: | human Topoisomerase II_lpha purified from HL-60 nuclear extract |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG2b |
| Product Type: | Primary Antibody |
| Shipping: | 4 |
| Size: | 100 µl |
| Species Reactivities: | Human |
| Status: | RUO |
| Storage: | 4℃ |
| Target: | Topoisomerase II α |
Applications: FCM, IC, IH, IP, WB
| FCM: | Optimal Dilution to be determined by end user. |
| ICC: | Optimal Dilution to be determined by end user. |
| IHC: | 1:200 (Heat treatment is necessary for paraffin em |
| IPP: | 10 uL/300 uL of cell extract from 5x106 |
| WB: | 1:200 (for chemiluminescence detection system) |
There are no references for Anti-DNA Topoisomerase IIα (Human) mAb at this time.
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