Solutions for Life Science & Diagnostics
Anti-Fas Ligand (CD178) (Human) mAb
Additional Information
Anti-Fas Ligand (CD178) (Human) mAb Specifications
| Background: | Fas ligand (FasL) is a type II-membrane protein, belonging to the tumor necrosis factor (TNF)/nerve growth factor (NGF) family. It is known that engagement of Fas (receptor of FasL) by FasL causes apoptotic cell death. FasL is predominantly expressed in activated T lymphocytes and Natural Killer (NK) cells and is expressed in the tissues of the immune-privilege sites such as the testis and eye. On the other hand, Fas is expressed in various tissues with abundant expression in the thymus, liver, heart, and kidney. Furthermore, a variety of cancers such as melanomas were found to express FasL in addition to Fas. It is said that Fas/FasL system is involved in the deletion of dangerous activated or autoreactive T and B cells and also plays a role in human diseases such as fulminant hepatitis, AIDS, cancers, and other diseases involving CTL-induced tissue destruction. Furthermore, FasL can be used as an immune-suppressive agent. Rejection of transplants is mediated by activated T cells that may express functional Fas. If a transplanted tissue is engineered to express FasL or is co-transplanted with FasL-expressing cells, the transplant may be tolerated. |
| Clonality: | Monoclonal |
| Clone Number: | 4A5 |
| Formulation: | This antibody is lyophilized form. Prepare a stock solution by dissolving the lyophilized antibody with 100 mL of distilled water. After reconstitution, IgG concentration should be 1 mg/mL in PBS (pH 7.2)/10 mg/mL sucrose. No preservative is contained. |
| Gene ID Human: | 355 |
| Gene ID Mouse: | 14102 |
| Host Species: | Hamster |
| Immunogen: | Recombinant Human Fas ligand |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG |
| Product Type: | Primary Antibody |
| Reactivity: | This antibody reacts with human soluble and membrane binding Fas Ligand. |
| Shipping: | 4 |
| Size: | 100 μg |
| Source: | This antibody was purified from culture supernatant by ammonium sulfate precipitation and affinity chromatography on protein A agarose. This hybridoma (clone 4A5) was established by fusion of mouse myeloma cell P3U1 with Armenian hamster lymphocyte immuni |
| Species Reactivities: | Human |
| Status: | RUO |
| Storage: | 4℃ |
| Target: | Fas |
Applications: ELISA, FCM
| ELISA: | Optimal Dilution to be determined by end user. |
| FCM: | 20 ug/mL |
1) Gilhar, A., et al., Am. J. Pathol. 168, 170-175 (2006) 2) Uno, K., et al., Blood 101, 3658-3667 (2003) 3) Zanin-Zhorov, A., et al., J. Immunol. 171, 5882-5889 (2003) 4) Durieu, I., et al., Thorax 54, 1093-1098 (1999) 5) Sato, K., et al., Br. J. Haemato
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