CircuLex™ Human soluble LOX-1/OLR1 ELISA Kit

Datasheet:

Background:

Lectin-like oxidized LDL receptor-1 (LOX-1), a 52-kD type II transmembrane receptor for oxidized low-density lipoproteins (ox-LDL) belonging to the C-type lectin family is present primarily on endothelial cells (1). Accumulating evidences indicate that oxLDL uptake through this receptor induces endothelial dysfunction. oxLDL binding to endothelial LOX-1 generates superoxide anions, decreases nitric oxide production, and activates NF-kB (2, 3). Endothelial LOX-1 expression is induced by various pro-inflammatory cytokines, such as TNF-alpha (4) and TGF-beta (5) as well as by pro-atherogenic factors, such as oxLDL and advanced glycation end products in vitro (6). This receptor is expressed in the aortas of hypertensive, diabetic, and hyperlipidemic animals (6-8) and is upregulated in early human atherosclerotic lesions (9). These results suggest that LOX-1 may be expressed locally and play important roles in atherogenesis by internalizing and degrading oxLDL and in inflammatory responses in vivo. LOX-1 can be cleaved from the cell surface and released as soluble LOX-1 (sLOX-1), and elevated sLOX-1 levels may be indicative of atherosclerotic plaque instability (10).

Size:

96 assays

Product Type:

Kit

Species Reactivities:

Human

Storage Temp. (°C):

4

Pictures:

CircuLex™ Human soluble LOX-1/OLR1 ELISA Kit

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CD5L /Sp? ELISA Kit: Cat# CY-8080;S100A12/EN-RAGE ELISA Kit: Cat# CY-8058;Anti-Human Sp?/CD5L (Lymphocyte antigen CD5-like): Cat# CY-P1036

References:

1. Sawamura T, Kume N, Aoyama T, Moriwaki H, Hoshikawa H, Aiba Y, Tanaka T, Miwa S, Katsura Y, Kita T, Masaki T. (1997) Nature 386: 73-77
2. Cominacini L, Pasini AF, Garbin U, Davoli A, Tosetti ML, Campagnola M, Rigoni A, Pastorino AM, Lo Cascio V, Sawamura T. (2000) J Biol Chem. 275: 12633ﾖ12638.
3. Cominacini L, Rigoni A, Pasini AF, Garbin U, Davoli A, Campagnola M, Pastorino AM, Lo Cascio U, Sawamura T. (2001) J Biol Chem. 276: 13750ﾖ13755.
4. Kume N, Murase T, Moriwaki H, Aoyama T, Sawamura T, Masaki T, Kita T. (1998) Circ Res. 83: 322ﾖ327.
5. Draude G and Lorenz R. L. (2000) Am J Physiol Heart Circ Physiol. 278: H1042ﾖH1048.
6. Nagase M, Hirose S, Sawamura T, Masaki T, Fujita T. (1997) Biochem Biophys Res Commun. 237: 496ﾖ498.
7. Chen M, Nagase M, Fujita T, Narumiya S, Masaki T, Sawamura T. (2001) Biochem Biophys Res Commun. 287: 962ﾖ968.
8. Chen M, Kakutani M, Minami M, Kataoka H, Kume N, Narumiya S, Kita T, Sawamura T. (2000) Arterioscler Thromb Vasc Biol. 20: 1107ﾖ1115.
9. Kataoka H, Kume N, Miyamoto S, Minami M, Moriwaki H, Murase T, Sawamura T, Masaki T, Hashimoto N, Kita T. (1999) Circulation 99: 3110 ﾖ3117.
10. Hayashida K, Kume N, Murase T, Minami M, Nakagawa D, Inada T, Tanaka M, Ueda A, Kominami G, Kambara H, Kimura T, Kita T. (2005) Circulation 112: 812-818.

Reactivity:

H

Intended Use:

For Research use only. Not for use in diagnostic procedure.

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