Solutions for Life Science & Diagnostics
CycLex Cellular UV DNA-Damage Detection Kit
Additional Information
CycLex Cellular UV DNA-Damage Detection Kit Specifications
| Background: | DNA damage in cells exposed to ultraviolet (UV) radiation plays significant roles in cell-cycle arrest, activation of DNA repair, cell killing, mutation, and neoplastic transformation. The major types of DNA damage induced by UVB (280-315 nm, component of sunlight) and by UVC (200-280 nm) are cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts (6-4PPs), which are formed between adjacent pyrimidine nucleotides on the same strand of DNA. Approximately 70-80% of UV-induced DNA damage is CPDs and the remaining is 6-4PPs and Dewar isomer of 6-4PPs. These types of DNA lesions are repaired by nucleotide excision repair (NER) system in normal human cells. Mori et al (10) have established monoclonal antibodies specific for CPDs or 6-4PPs. These antibodies enable one to quantitate photoproducts in DNA purified from cultured cells or from the skin epidermis using an enzyme-linked immunosorbent assay (ELISA) and to visualize and measure photoproducts in DNA in cultured cells or the skin using indirect immunofluorescence (IIF). This technology would contribute to understanding of molecular mechanisms of cellular responses to UV and DNA damage in the photobiology and the pigment cell biology. A rapid and convenient method for evaluating the formation of cyclobutane pyrimidine dimers (CPDs) in a cell after UV-irradiation was developed which detects CPDs adducts on the genomic DNA by means of anti-CPDs antibody in a cellular ELISA format. It has been shown that a precise evaluation of CPDs formation, could be performed by the measurement of CPDs adducts in purified genomic DNA by ELISA. In addition, there is a good reverse proportion between the cellular CPDs ELISA value and ability of nucleotide excision repair (NER) when the cell are incubate for several hours after UV-irradiation for repair as shown for a variety of NER deficient cell lines, such as xeroderma patient-derived cell lines. The CycLex® Cellular UV DNA-Damage Detection Kit can be used in many different in vitro cell systems without any purification step of genomic DNA. |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Product Type: | Kit |
| Shipping: | 4 |
| Size: | 200 assay |
| Status: | RUO |
| Storage: | 4 |
| Target: | Cellular UV DNA-Damage Detection |
Applications:
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