- Diagnostic Products
- Research Products
|Background:||Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a novel and convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm. As the D2R is more cell-permeable than other fluorometric caspase substrates, activation of caspases can easily be measured in intact cells by flow cytometry.|
|Regulatory Statement:||For Research Use Only. Not for use in diagnostic procedures.|
|Target:||Apoptosis Detection Kit|
There are no references for CaspSCREEN Flow Cytometric Apoptosis Detection Kit at this time.