Solutions for Life Science & Diagnostics
caspase-5 Fluorometric Protease Assay Kit
Additional Information
caspase-5 Fluorometric Protease Assay Kit Specifications
| Background: | Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-5 Fluorometric Assay Kits provide a simple and convenient means for assaying the activity of caspase-1 and related caspases that recognize the sequence WEHD. The assay is based on detection of cleavage of substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity |
| Clonality: | N/A |
| Clone Number: | N/A |
| Host Species: | N/A |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Product Type: | Kit |
| Shipping: | 4 |
| Size: | 200 assays |
| Status: | RUO |
| Storage: | -20 |
| Target: | caspase-5 |
Applications:
There are no references for caspase-5 Fluorometric Protease Assay Kit at this time.
