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|Background:||Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9/Mch6 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (Î»max = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (Î»max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.|
|Regulatory Statement:||For Research Use Only. Not for use in diagnostic procedures.|
There are no references for caspase-9/Mch6 Fluorometric Assay Kit at this time.