Solutions for Life Science & Diagnostics
caspase-9/Mch6 Fluorometric Assay Kit
Additional Information
caspase-9/Mch6 Fluorometric Assay Kit Specifications
| Background: | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9/Mch6 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity. |
| Clonality: | N/A |
| Clone Number: | N/A |
| Host Species: | N/A |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Product Type: | Kit |
| Shipping: | 4 |
| Size: | 200 assays |
| Status: | RUO |
| Storage: | -20 |
| Target: | caspase-9/Mch6 |
Applications:
There are no references for caspase-9/Mch6 Fluorometric Assay Kit at this time.
