The plasmid DNA encodes a monomeric version of the fluorescent protein CoralHueﾮ Azami-Green (AG). CoralHueﾮ AG has been cloned from the stony coral, whose Japanese name is ﾓAzami-Sangoﾔ. It absorbs light maximally at 492 nm and emits green light at 505 nm. Wild-type CoralHueﾮ AG rapidly matures to form a tetrameric complex. CoralHueﾮ AG has been carefully engineered to form a monomer, CoralHueﾮ Monomeric Azami-Green (mAG1) that maintains the brilliance and pH stability of the parent protein. CoralHueﾮ mAG1 can be used to label proteins or subcellular structures. CoralHueﾮ hmAG1 sequence is codon-optimized for higher expression in mammalian cells. This plasmid has the flexible linker between the fluorescent protein and the multi cloning site.