Angiogenesis
Apoptosis
Autophagy & Proteolysis
Cancer & Cell Proliferation
Cell Surface Antigens
Cellular Stress
Cytokines & Growth Factors
Epigenetics
Epitope Tags
Fluorescent Proteins
GPCR
In Vitro Diagnostics
Inflammation & Immunology
Metabolism
Molecular Biology
Neuroscience
Signal Transduction
Solute Carrier
Virology
Other
Product Catalog |
Cathepsin S Activity Assay KitCode No: JM-K144-100 Background:
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-S Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The cathepsin-S assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity.
Size:
100 assays
Storage Temp. (°C):
-20
Shipping Temp. (°C):
4
Intended Use:
For Research use only. Not for use in diagnostic procedure. |
