Biotinylated Protein G

Datasheet:

Background:

Protein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies.

Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.

The Protein G-Biotin is using purified recombinant protein G biotinylated with ~2 biotins per protein G molecule.

Size:

100 μg

Product Type:

Protein

Storage Temp. (°C):

-20

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Purity:

>98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 EU/mg of Protein G.

Intended Use:

For Research use only. Not for use in diagnostic procedure.

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