- Diagnostic Products
- Research Products
|Background:||Ready-to-use fluorometric substrate for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD. Caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. # BV-1067-3) and 2X Reaction Buffer (Cat. # BV-1068-80) and DTT (Cat. # JM-1201-1) for caspase assays are also available separately.|
|Regulatory Statement:||For Research Use Only. Not for use in diagnostic procedures.|
There are no references for caspase-9 Fluorometric Substrate LEHD-AFC at this time.