Solutions for Life Science & Diagnostics
Anti-HA-tag pAb-Agarose
Additional Information
Anti-HA-tag pAb-Agarose Specifications
| Background: | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. With this technique, the gene expression can be easily monitored by Western blotting, immunoprecipitation and immunofluorescence. Amino acid sequences that are commonly used for epitope tagging include peptide sequences corresponding to portions of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein. |
| Clonality: | Polyclonal |
| Conjugate: | Agarose |
| Formulation: | 540 mg of anti-HA Tag polyclonal antibody covalently coupled to 200 mL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 mL. |
| Host Species: | Rabbit |
| Immunogen: | YPYDVPDYA (HA-tag)-KLH |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
| Isotype: | IgG |
| Product Type: | Primary Antibody |
| Shipping: | 4 |
| Size: | 400 uL |
| Status: | RUO |
| Storage: | 4℃ |
| Target: | HA |
Applications: IP
| IPP: | 20 μL of gel slurry |
1) Sun, P., et al., Genes Cells 11, 1097-1113 (2006) 2) Arao, Y., et al., Mol. Endocrinol 18, 2255-2267 (2004) 3) Myojin, R., et al., Biol. Reprod 71, 926-932 (2004) 4) Zhang, A., et al., J. Biol. Chem. 279, 33799-33805 (2004) 5) Murayama, Y., et al., J.
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