Abstract

MicroRNAs (miRNAs) are genomically encoded non-coding RNAs that take part in controlling gene expression. Mature miRNAs are integrated onto RNA-induced silencing complex (RISC) which targets messenger RNA (mRNA) to regulate their translation. mRNAs are also regulated by RNA binding proteins (RBPs). RBPs have been reported to bind with mRNAs that encode functionally related proteins. By taking advantage of RISC and RBPs, a RIP-Assay Kit for microRNAs was developed to study miRNAs by two approaches. Approach I employs a specific antibody against Argonaute (AGO2), which is a core component of RISK, to comprehensively isolate and identify miRNAs that loaded on RISC. Approach II is based on a broad range of specific antibodies against RBPs to isolate and identify functionally related mRNAs. Since miRNAs are immunoprecipitated together with these mRNAs, this approach is useful to identify and isolate miRNAs that regulate functionally related mRNAs. 

References:

1) Hammond S, Bernstein E, Beach D, Hannon G (2000). "An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells". Nature 404 (6775): 293–6.

2) Keene JD (2007). RNA regulons: coordination of post-transcriptional events. Nat. Rev. Genet. 8:533-543.

File: 

Poster for 2011 microRNA.pdf

FID:14795 | PATH:sites/default/files/Poster for 2011 microRNA.pdf