Abstract

Post-transcriptional regulation of gene expression plays important roles in most cellular processes such as development and metabolism as well as many diseases such as cancer. RIP-Chip and RIP-Seq have been widely used to identify the functionally related mRNAs by taking the advantage of RNA binding proteins (RBPs) and their specific antibodies. Besides these tools, a discovery approach based on complementary binding of RNA is also required to characterize RBPs assembled on any RNA of interest. RiboTrap employs 5-bromo-UTP (BrU) labeled RNA as ‘bait’ and a BrdU antibody for immunnoprecipitation to identify RBPs that are associated with the RNA of interest such as mRNA. 

Reference:

Keene, JD., et al., Methods Mol. Biol. (2008) 419:69-91

File: 

Poster for 2011 RipTrap.pdf

FID:14797 | PATH:sites/default/files/Poster for 2011 RipTrap_0.pdf