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Product Catalog |
NMNAT2 (Nicotinamide mononucleotide adenylyltransferase 2)Human, recombinant protein expressed in E. coli., ActiveBackground:
Nicotinamide mononucleotide adenylyltransferase (NMNAT) (EC2.7.7.1) is a central enzyme in NAD+ biosynthesis, transferring the adenylyl moiety of ATP to nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) resulting in the formation of NAD+ or NaAD+ and the release of pyrophosphate. As this reaction is reversible, the enzyme may in principle be used to form ATP and NMN from NAD+ and pyrophosphate.
Size:
50 ?g
Application:
Product Type:
Reagent
Species Reactivities:
H
Storage Temp. (°C):
-70
Shipping Temp. (°C):
Dry Ice References:
1. Raffaelli, N.; Sorci, L.; Amici, A.; Emanuelli, M.; Mazzola, F.; Magni, G. : Biochem. Biophys. Res. Commun. 297: 835-840, 2002. 2. Seki, N.; Ohira, M.; Nagase, T.; Ishikawa, K.; Miyajima, N.; Nakajima, D.; Nomura, N.; Ohara, O. : DNA Res. 4: 345-349, 1997. 3. Yalowitz, J. A.; Xiao, S.; Biju, M. P.; Antony, A. C.; Cummings, O. W.; Deeg, M. A.; Jayaram, H. N. : Biochem. J. 377: 317-326, 2004. 4. Zhang, X.; Kurnasov, O. V.; Karthikeyan, S.; Grishin, N. V.; Osterman, A. L.; Zhang, H. : J. Biol. Chem. 278: 13503-13511, 2003. 5. J Gilley and MP Coleman: PLoS Biol. 8: e1000300, 2010
Intended Use:
For Research use only. Not for use in diagnostic procedure. CircuLex™ Human soluble LOX-1/OLR1 ELISA KitBackground:
Lectin-like oxidized LDL receptor-1 (LOX-1), a 52-kD type II transmembrane receptor for oxidized low-density lipoproteins (ox-LDL) belonging to the C-type lectin family is present primarily on endothelial cells (1). Accumulating evidences indicate that oxLDL uptake through this receptor induces endothelial dysfunction. oxLDL binding to endothelial LOX-1 generates superoxide anions, decreases nitric oxide production, and activates NF-kB (2, 3). Endothelial LOX-1 expression is induced by various pro-inflammatory cytokines, such as TNF-alpha (4) and TGF-beta (5) as well as by pro-atherogenic factors, such as oxLDL and advanced glycation end products in vitro (6). This receptor is expressed in the aortas of hypertensive, diabetic, and hyperlipidemic animals (6-8) and is upregulated in early human atherosclerotic lesions (9). These results suggest that LOX-1 may be expressed locally and play important roles in atherogenesis by internalizing and degrading oxLDL and in inflammatory responses in vivo. LOX-1 can be cleaved from the cell surface and released as soluble LOX-1 (sLOX-1), and elevated sLOX-1 levels may be indicative of atherosclerotic plaque instability (10).
Size:
96 assays
Application:
Product Type:
Kit
Species Reactivities:
H
Storage Temp. (°C):
4 References:
1. Sawamura T, Kume N, Aoyama T, Moriwaki H, Hoshikawa H, Aiba Y, Tanaka T, Miwa S, Katsura Y, Kita T, Masaki T. (1997) Nature 386: 73-77
Intended Use:
For Research use only. Not for use in diagnostic procedure. NAD+/NADH Colorimetric Assay KitBackground:
Nicotinamide adenine dinucleotide (NAD+) as well as nicotinamide adenine dinucleotide phosphate (NADP+) is an important cofactor found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It has been reported that NAD+ metabolism regulates important biological effects including life span. NAD+, through poly-ADP-ribosyl polymerase (PARP), mono-ADP- ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation in the presence of NAD+. These enzymes are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling, suggesting that either the enzymes could be an important therapeutical target for cancer, diabetes atherosclerosis and so on.
Size:
100 assays
Application:
Product Type:
Kit
Species Reactivities:
H
Storage Temp. (°C):
-70
Shipping Temp. (°C):
Dry Ice References:
1. Ziegenhorn J, Senn M, Bucher T. (1976) Molar absorptivities of beta-NADH and beta-NADPH. Clin 2. Matsumura, H. and Miyachi S (1980) Cycling assay for nicotinamide adenine dinucleotides. Methods Enzymol. 69: 465-470. 3. Zerez CR, Lee SJ, Tanaka KR (1987) Spectrophotometric determination of oxidized and reduced pyridine nucleotides in erythrocytes using a single extraction procedure. Anal Biochem. 164: 367-73 4. Zhao, Z, Hu, X and Ross CW (1987). Comparison of Tissue Preparation Methods for Assay of Nicotinamide Coenzymes. Plant Physiol. 84: 987-988. 5 Umemura, K and Kimura, H (2005) Determination of oxidized and reduced nicotinamide adenine dinucleotide in cell monolayers using a single extraction procedure and a spectrophotometric assay. Anal Biochem. 338: 131-5. 6. Hasmann, M and Schemainda, I (2003) FK866, a Highly Specific Noncompetitive Inhibitor of Nicotinamide Phosphoribosyltransferase, Represents a Novel Mechanism for Induction of Tumor Cell Apoptosis. Cancer Research 63: 7436-7442, 2003 7.Vilcheze, C et al. (2005). Altered NADH/NAD+ Ratio Mediates Coresistance to Isoniazid and Ethionamide in Mycobacteria. Antimicrobial Agents and Chemotherapy. 49: 708-720. 8. Kimura N, Fukuwatari T, Sasaki R, Shibata K. (2006) Comparison of metabolic fates of nicotinamide, NAD+ and NADH administered orally and intraperitoneally; characterization of oral NADH. J Nutr Sci Vitaminol. (Tokyo) 52: 142. 9. O'Donnell JM, et al. (2004) Limited transfer of cytosolic NADH into mitochondria at high cardiac workload. Am J Physiol Heart Circ Physiol. 286: H2237. 10. Richard A. et al. (2008) Characterization of NAD Uptake in Mammalian Cells J. Biol. Chem. 283: 6367-6374. 11. Rongvaux, A et al. (2008) Nicotinamide Phosphoribosyl Transferase/Pre-B Cell Colony-Enhancing Factor/Visfatin Is Required for Lymphocyte Development and Cellular Resistance to Genotoxic Stress J. Immunol. 181: 4685-4695 12. Pogrebniak, A et al. (2006) Chemopotentiating effects of a novel NAD biosynthesis inhibitor, FK866, in combination with antineoplastic agents. Eur J Med Res. 11: 313-21.
Intended Use:
For Research use only. Not for use in diagnostic procedure. anti-GM130Background:
The Golgi apparatus is a eukaryotic organelle, which is mainly devoted to processing the proteins synthesized in the endoplasmic reticulum (ER). GM130 is a member of the golgin family of coiled-coil proteins that localizes predominantly to the cis-Golgi. GM130 might participate in ER-Golgi traffic.
Size:
100 μL
Application:
Product Type:
Primary Antibody
Host Species:
Rabbit
Species Reactivities:
H
Isotype:
Ig (aff.)
Storage Temp. (°C):
-20
Shipping Temp. (°C):
4 Pictures:
   References:
Diao, A., et al., J. Biol. Chem. 283, 6957-6967 (2008)
Intended Use:
For Research use only. Not for use in diagnostic procedure. anti-EEA1Background:
The early endosome is a cellular compartment inside eukaryotic cells. It is receiving endocytosed material and sorting them for vesicular transport to late endosomes and lysosomes or for recycling to the plasma membrane. EEA1 (Early Endosome Antigen1) is a 170 kDa coiled-coil protein, which is required for vesicular transport of proteins through early endosomes. It binds membrane lipids through its FYVE domain.
Size:
100 μL
Application:
Product Type:
Primary Antibody
Host Species:
Rabbit
Species Reactivities:
H
Species Reactivities:
M
Species Reactivities:
R
Isotype:
Ig (aff.)
Storage Temp. (°C):
-20
Shipping Temp. (°C):
4 Pictures:
   References:
Gaullier, J. M., et al., J. Biol. Chem. 275, 24595-24600 (2000)
Intended Use:
For Research use only. Not for use in diagnostic procedure. anti-CalnexinBackground:
The endoplasmic reticulum (ER) is a eukaryotic organelle, which serves many general functions, including the facilitation of protein folding. Calnexin is a 90 kDa integral membrane protein of the ER. Calnexin is one of the chaperone proteins, which play a major role in the quality control of the ER by the retention of incorrectly folded proteins.
Size:
100 μL
Application:
Product Type:
Primary Antibody
Host Species:
Rabbit
Species Reactivities:
H
Isotype:
Ig (aff.)
Storage Temp. (°C):
-20
Shipping Temp. (°C):
4 Pictures:
  
Intended Use:
For Research use only. Not for use in diagnostic procedure. anti-KDELBackground:
The KDEL (lys-asp-glu-leu) sequence is most common endoplasmic reticulum (ER) retention signal. The ER resident proteins, which should be located in the ER, have this retention motif, and this retention is mediated by KDEL receptor. This antibody can specifically detect GRP78 (78 kDa protein) and GRP94 (94 kDa protein) including a KDEL sequence by WB.
Size:
100 μL
Application:
Product Type:
Primary Antibody
Host Species:
Rabbit
Species Reactivities:
H
Species Reactivities:
M
Isotype:
Ig (aff.)
Storage Temp. (°C):
-20
Shipping Temp. (°C):
4 Pictures:
  References:
Pelham, H. R., EMBO J. 7, 913-918 (1988)
Intended Use:
For Research use only. Not for use in diagnostic procedure. anti-V5-tag HRP-DirecTBackground:
HRP-DirecT is a series of HRP conjugated primary antibodies developed by MBL.
Size:
100 μL
Application:
Product Type:
Primary Antibody
Host Species:
Rabbit
Isotype:
Ig (aff.)
Storage Temp. (°C):
4
Shipping Temp. (°C):
4
Intended Use:
For Research use only. Not for use in diagnostic procedure. anti-DDDDK-tag HRP-DirecTBackground:
HRP-DirecT is a series of HRP conjugated primary antibodies developed by MBL.
Size:
100 μL
Application:
Product Type:
Primary Antibody
Host Species:
Rabbit
Isotype:
Ig (aff.)
Storage Temp. (°C):
4
Shipping Temp. (°C):
4
Intended Use:
For Research use only. Not for use in diagnostic procedure. anti-RFP HRP-DirecTBackground:
HRP-DirecT Series
Size:
100 μL
Application:
Product Type:
Primary Antibody
Host Species:
Rabbit
Isotype:
IgG
Storage Temp. (°C):
4
Shipping Temp. (°C):
4
Intended Use:
For Research use only. Not for use in diagnostic procedure. |
